rstoolbox.components.DesignSeries.generate_wt_reversions

DesignSeries.generate_wt_reversions(seqID, key_residues=None)

Generate all variant that revert decoy sequences to the reference_sequence.

Expand the selected sequences by generating all the combinatorial options to revert to the reference (WT) sequence.

Alters the names of the designs in description column.

Warning

This is a computationaly expensive function. Take this in consideration when trying to run it. When trying to revert too many mutations, it can run out of memory.

Parameters:
  • seqID (str) – Identifier of the sequence of interest.
  • key_residues (Union[int, list() of int, str, Selection]) – Residues of interest
Returns:

DesignFrame

Example

In [1]: from rstoolbox.io import parse_rosetta_file
   ...: import pandas as pd
   ...: pd.set_option('display.width', 1000)
   ...: pd.set_option('display.max_columns', 500)
   ...: df = parse_rosetta_file("../rstoolbox/tests/data/input_2seq.minisilent.gz",
   ...:                         {'scores': ['score', 'description'], 'sequence': 'B'})
   ...: df.add_reference_sequence('B', df.get_sequence('B').values[0])
   ...: key_res = [3, 5, 8, 12, 15, 19, 25, 27]
   ...: df.iloc[1].generate_wt_reversions('B', key_res).identify_mutants('B')
   ...: 
Out[1]: 
                                                                                                              sequence_B                           description                                                                                                                                                                                                                                                                                                                                                                                         mutants_B                                                                                                                                                                                                                          mutant_positions_B  mutant_count_B
0   PKPEEAMREAYKLIKKYMLKAQKEAQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002        T1P,R2K,R7M,E8R,R9E,W11Y,R12K,A14I,E15K,I16K,A17Y,R19L,G21A,W22Q,E23K,H25A,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R  1,2,7,8,9,11,12,14,15,16,17,19,21,22,23,25,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116  75            
1   PKPEEAMEEAYRLIEKYMRKAQKEHQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0001  T1P,R2K,R7M,R9E,W11Y,A14I,I16K,A17Y,G21A,W22Q,E23K,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                               1,2,7,9,11,14,16,17,21,22,23,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116                   69            
2   PKPEEAMEEAYRLIEKYMRKAQKEHQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0002  T1P,R2K,R7M,R9E,W11Y,A14I,I16K,A17Y,G21A,W22Q,E23K,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                          1,2,7,9,11,14,16,17,21,22,23,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116                70            
3   PKPEEAMEEAYRLIEKYMRKAQKEAQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0003  T1P,R2K,R7M,R9E,W11Y,A14I,I16K,A17Y,G21A,W22Q,E23K,H25A,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                          1,2,7,9,11,14,16,17,21,22,23,25,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116                70            
4   PKPEEAMEEAYRLIEKYMRKAQKEAQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0004  T1P,R2K,R7M,R9E,W11Y,A14I,I16K,A17Y,G21A,W22Q,E23K,H25A,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                     1,2,7,9,11,14,16,17,21,22,23,25,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116             71            
5   PKPEEAMEEAYRLIEKYMLKAQKEHQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0005  T1P,R2K,R7M,R9E,W11Y,A14I,I16K,A17Y,R19L,G21A,W22Q,E23K,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                          1,2,7,9,11,14,16,17,19,21,22,23,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116                70            
6   PKPEEAMEEAYRLIEKYMLKAQKEHQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0006  T1P,R2K,R7M,R9E,W11Y,A14I,I16K,A17Y,R19L,G21A,W22Q,E23K,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                     1,2,7,9,11,14,16,17,19,21,22,23,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116             71            
7   PKPEEAMEEAYRLIEKYMLKAQKEAQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0007  T1P,R2K,R7M,R9E,W11Y,A14I,I16K,A17Y,R19L,G21A,W22Q,E23K,H25A,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                     1,2,7,9,11,14,16,17,19,21,22,23,25,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116             71            
8   PKPEEAMEEAYRLIEKYMLKAQKEAQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0008  T1P,R2K,R7M,R9E,W11Y,A14I,I16K,A17Y,R19L,G21A,W22Q,E23K,H25A,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                1,2,7,9,11,14,16,17,19,21,22,23,25,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116          72            
9   PKPEEAMEEAYRLIKKYMRKAQKEHQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0009  T1P,R2K,R7M,R9E,W11Y,A14I,E15K,I16K,A17Y,G21A,W22Q,E23K,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                          1,2,7,9,11,14,15,16,17,21,22,23,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116                70            
10  PKPEEAMEEAYRLIKKYMRKAQKEHQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0010  T1P,R2K,R7M,R9E,W11Y,A14I,E15K,I16K,A17Y,G21A,W22Q,E23K,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                     1,2,7,9,11,14,15,16,17,21,22,23,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116             71            
11  PKPEEAMEEAYRLIKKYMRKAQKEAQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0011  T1P,R2K,R7M,R9E,W11Y,A14I,E15K,I16K,A17Y,G21A,W22Q,E23K,H25A,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                     1,2,7,9,11,14,15,16,17,21,22,23,25,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116             71            
12  PKPEEAMEEAYRLIKKYMRKAQKEAQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0012  T1P,R2K,R7M,R9E,W11Y,A14I,E15K,I16K,A17Y,G21A,W22Q,E23K,H25A,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                1,2,7,9,11,14,15,16,17,21,22,23,25,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116          72            
13  PKPEEAMEEAYRLIKKYMLKAQKEHQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0013  T1P,R2K,R7M,R9E,W11Y,A14I,E15K,I16K,A17Y,R19L,G21A,W22Q,E23K,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                     1,2,7,9,11,14,15,16,17,19,21,22,23,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116             71            
14  PKPEEAMEEAYRLIKKYMLKAQKEHQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0014  T1P,R2K,R7M,R9E,W11Y,A14I,E15K,I16K,A17Y,R19L,G21A,W22Q,E23K,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                1,2,7,9,11,14,15,16,17,19,21,22,23,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116          72            
15  PKPEEAMEEAYRLIKKYMLKAQKEAQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0015  T1P,R2K,R7M,R9E,W11Y,A14I,E15K,I16K,A17Y,R19L,G21A,W22Q,E23K,H25A,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                1,2,7,9,11,14,15,16,17,19,21,22,23,25,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116          72            
16  PKPEEAMEEAYRLIKKYMLKAQKEAQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0016  T1P,R2K,R7M,R9E,W11Y,A14I,E15K,I16K,A17Y,R19L,G21A,W22Q,E23K,H25A,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R           1,2,7,9,11,14,15,16,17,19,21,22,23,25,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116       73            
17  PKPEEAMEEAYKLIEKYMRKAQKEHQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0017  T1P,R2K,R7M,R9E,W11Y,R12K,A14I,I16K,A17Y,G21A,W22Q,E23K,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                          1,2,7,9,11,12,14,16,17,21,22,23,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116                70            
18  PKPEEAMEEAYKLIEKYMRKAQKEHQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0018  T1P,R2K,R7M,R9E,W11Y,R12K,A14I,I16K,A17Y,G21A,W22Q,E23K,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                     1,2,7,9,11,12,14,16,17,21,22,23,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116             71            
19  PKPEEAMEEAYKLIEKYMRKAQKEAQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0019  T1P,R2K,R7M,R9E,W11Y,R12K,A14I,I16K,A17Y,G21A,W22Q,E23K,H25A,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                     1,2,7,9,11,12,14,16,17,21,22,23,25,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116             71            
20  PKPEEAMEEAYKLIEKYMRKAQKEAQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0020  T1P,R2K,R7M,R9E,W11Y,R12K,A14I,I16K,A17Y,G21A,W22Q,E23K,H25A,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                1,2,7,9,11,12,14,16,17,21,22,23,25,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116          72            
21  PKPEEAMEEAYKLIEKYMLKAQKEHQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0021  T1P,R2K,R7M,R9E,W11Y,R12K,A14I,I16K,A17Y,R19L,G21A,W22Q,E23K,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                     1,2,7,9,11,12,14,16,17,19,21,22,23,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116             71            
22  PKPEEAMEEAYKLIEKYMLKAQKEHQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0022  T1P,R2K,R7M,R9E,W11Y,R12K,A14I,I16K,A17Y,R19L,G21A,W22Q,E23K,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                1,2,7,9,11,12,14,16,17,19,21,22,23,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116          72            
23  PKPEEAMEEAYKLIEKYMLKAQKEAQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0023  T1P,R2K,R7M,R9E,W11Y,R12K,A14I,I16K,A17Y,R19L,G21A,W22Q,E23K,H25A,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                1,2,7,9,11,12,14,16,17,19,21,22,23,25,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116          72            
24  PKPEEAMEEAYKLIEKYMLKAQKEAQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0024  T1P,R2K,R7M,R9E,W11Y,R12K,A14I,I16K,A17Y,R19L,G21A,W22Q,E23K,H25A,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R           1,2,7,9,11,12,14,16,17,19,21,22,23,25,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116       73            
25  PKPEEAMEEAYKLIKKYMRKAQKEHQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0025  T1P,R2K,R7M,R9E,W11Y,R12K,A14I,E15K,I16K,A17Y,G21A,W22Q,E23K,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                     1,2,7,9,11,12,14,15,16,17,21,22,23,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116             71            
26  PKPEEAMEEAYKLIKKYMRKAQKEHQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0026  T1P,R2K,R7M,R9E,W11Y,R12K,A14I,E15K,I16K,A17Y,G21A,W22Q,E23K,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                1,2,7,9,11,12,14,15,16,17,21,22,23,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116          72            
27  PKPEEAMEEAYKLIKKYMRKAQKEAQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0027  T1P,R2K,R7M,R9E,W11Y,R12K,A14I,E15K,I16K,A17Y,G21A,W22Q,E23K,H25A,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                1,2,7,9,11,12,14,15,16,17,21,22,23,25,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116          72            
28  PKPEEAMEEAYKLIKKYMRKAQKEAQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0028  T1P,R2K,R7M,R9E,W11Y,R12K,A14I,E15K,I16K,A17Y,G21A,W22Q,E23K,H25A,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R           1,2,7,9,11,12,14,15,16,17,21,22,23,25,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116       73            
29  PKPEEAMEEAYKLIKKYMLKAQKEHQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0029  T1P,R2K,R7M,R9E,W11Y,R12K,A14I,E15K,I16K,A17Y,R19L,G21A,W22Q,E23K,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                1,2,7,9,11,12,14,15,16,17,19,21,22,23,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116          72            
..                                                                                                                   ...                                   ...                                                                                                                                                                                                                                                                                                                                                                                 ...                                                                                                                                                                                                                                               ...          ..            
34  PKPEEAMREAYRLIEKYMRKAQKEHQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0034  T1P,R2K,R7M,E8R,R9E,W11Y,A14I,I16K,A17Y,G21A,W22Q,E23K,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                      1,2,7,8,9,11,14,16,17,21,22,23,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116              71            
35  PKPEEAMREAYRLIEKYMRKAQKEAQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0035  T1P,R2K,R7M,E8R,R9E,W11Y,A14I,I16K,A17Y,G21A,W22Q,E23K,H25A,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                      1,2,7,8,9,11,14,16,17,21,22,23,25,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116              71            
36  PKPEEAMREAYRLIEKYMRKAQKEAQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0036  T1P,R2K,R7M,E8R,R9E,W11Y,A14I,I16K,A17Y,G21A,W22Q,E23K,H25A,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                 1,2,7,8,9,11,14,16,17,21,22,23,25,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116           72            
37  PKPEEAMREAYRLIEKYMLKAQKEHQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0037  T1P,R2K,R7M,E8R,R9E,W11Y,A14I,I16K,A17Y,R19L,G21A,W22Q,E23K,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                      1,2,7,8,9,11,14,16,17,19,21,22,23,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116              71            
38  PKPEEAMREAYRLIEKYMLKAQKEHQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0038  T1P,R2K,R7M,E8R,R9E,W11Y,A14I,I16K,A17Y,R19L,G21A,W22Q,E23K,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                 1,2,7,8,9,11,14,16,17,19,21,22,23,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116           72            
39  PKPEEAMREAYRLIEKYMLKAQKEAQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0039  T1P,R2K,R7M,E8R,R9E,W11Y,A14I,I16K,A17Y,R19L,G21A,W22Q,E23K,H25A,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                 1,2,7,8,9,11,14,16,17,19,21,22,23,25,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116           72            
40  PKPEEAMREAYRLIEKYMLKAQKEAQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0040  T1P,R2K,R7M,E8R,R9E,W11Y,A14I,I16K,A17Y,R19L,G21A,W22Q,E23K,H25A,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R            1,2,7,8,9,11,14,16,17,19,21,22,23,25,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116        73            
41  PKPEEAMREAYRLIKKYMRKAQKEHQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0041  T1P,R2K,R7M,E8R,R9E,W11Y,A14I,E15K,I16K,A17Y,G21A,W22Q,E23K,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                      1,2,7,8,9,11,14,15,16,17,21,22,23,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116              71            
42  PKPEEAMREAYRLIKKYMRKAQKEHQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0042  T1P,R2K,R7M,E8R,R9E,W11Y,A14I,E15K,I16K,A17Y,G21A,W22Q,E23K,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                 1,2,7,8,9,11,14,15,16,17,21,22,23,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116           72            
43  PKPEEAMREAYRLIKKYMRKAQKEAQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0043  T1P,R2K,R7M,E8R,R9E,W11Y,A14I,E15K,I16K,A17Y,G21A,W22Q,E23K,H25A,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                 1,2,7,8,9,11,14,15,16,17,21,22,23,25,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116           72            
44  PKPEEAMREAYRLIKKYMRKAQKEAQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0044  T1P,R2K,R7M,E8R,R9E,W11Y,A14I,E15K,I16K,A17Y,G21A,W22Q,E23K,H25A,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R            1,2,7,8,9,11,14,15,16,17,21,22,23,25,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116        73            
45  PKPEEAMREAYRLIKKYMLKAQKEHQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0045  T1P,R2K,R7M,E8R,R9E,W11Y,A14I,E15K,I16K,A17Y,R19L,G21A,W22Q,E23K,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                 1,2,7,8,9,11,14,15,16,17,19,21,22,23,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116           72            
46  PKPEEAMREAYRLIKKYMLKAQKEHQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0046  T1P,R2K,R7M,E8R,R9E,W11Y,A14I,E15K,I16K,A17Y,R19L,G21A,W22Q,E23K,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R            1,2,7,8,9,11,14,15,16,17,19,21,22,23,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116        73            
47  PKPEEAMREAYRLIKKYMLKAQKEAQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0047  T1P,R2K,R7M,E8R,R9E,W11Y,A14I,E15K,I16K,A17Y,R19L,G21A,W22Q,E23K,H25A,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R            1,2,7,8,9,11,14,15,16,17,19,21,22,23,25,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116        73            
48  PKPEEAMREAYRLIKKYMLKAQKEAQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0048  T1P,R2K,R7M,E8R,R9E,W11Y,A14I,E15K,I16K,A17Y,R19L,G21A,W22Q,E23K,H25A,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R       1,2,7,8,9,11,14,15,16,17,19,21,22,23,25,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116     74            
49  PKPEEAMREAYKLIEKYMRKAQKEHQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0049  T1P,R2K,R7M,E8R,R9E,W11Y,R12K,A14I,I16K,A17Y,G21A,W22Q,E23K,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                      1,2,7,8,9,11,12,14,16,17,21,22,23,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116              71            
50  PKPEEAMREAYKLIEKYMRKAQKEHQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0050  T1P,R2K,R7M,E8R,R9E,W11Y,R12K,A14I,I16K,A17Y,G21A,W22Q,E23K,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                 1,2,7,8,9,11,12,14,16,17,21,22,23,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116           72            
51  PKPEEAMREAYKLIEKYMRKAQKEAQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0051  T1P,R2K,R7M,E8R,R9E,W11Y,R12K,A14I,I16K,A17Y,G21A,W22Q,E23K,H25A,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                 1,2,7,8,9,11,12,14,16,17,21,22,23,25,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116           72            
52  PKPEEAMREAYKLIEKYMRKAQKEAQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0052  T1P,R2K,R7M,E8R,R9E,W11Y,R12K,A14I,I16K,A17Y,G21A,W22Q,E23K,H25A,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R            1,2,7,8,9,11,12,14,16,17,21,22,23,25,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116        73            
53  PKPEEAMREAYKLIEKYMLKAQKEHQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0053  T1P,R2K,R7M,E8R,R9E,W11Y,R12K,A14I,I16K,A17Y,R19L,G21A,W22Q,E23K,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                 1,2,7,8,9,11,12,14,16,17,19,21,22,23,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116           72            
54  PKPEEAMREAYKLIEKYMLKAQKEHQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0054  T1P,R2K,R7M,E8R,R9E,W11Y,R12K,A14I,I16K,A17Y,R19L,G21A,W22Q,E23K,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R            1,2,7,8,9,11,12,14,16,17,19,21,22,23,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116        73            
55  PKPEEAMREAYKLIEKYMLKAQKEAQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0055  T1P,R2K,R7M,E8R,R9E,W11Y,R12K,A14I,I16K,A17Y,R19L,G21A,W22Q,E23K,H25A,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R            1,2,7,8,9,11,12,14,16,17,19,21,22,23,25,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116        73            
56  PKPEEAMREAYKLIEKYMLKAQKEAQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0056  T1P,R2K,R7M,E8R,R9E,W11Y,R12K,A14I,I16K,A17Y,R19L,G21A,W22Q,E23K,H25A,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R       1,2,7,8,9,11,12,14,16,17,19,21,22,23,25,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116     74            
57  PKPEEAMREAYKLIKKYMRKAQKEHQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0057  T1P,R2K,R7M,E8R,R9E,W11Y,R12K,A14I,E15K,I16K,A17Y,G21A,W22Q,E23K,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R                 1,2,7,8,9,11,12,14,15,16,17,21,22,23,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116           72            
58  PKPEEAMREAYKLIKKYMRKAQKEHQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0058  T1P,R2K,R7M,E8R,R9E,W11Y,R12K,A14I,E15K,I16K,A17Y,G21A,W22Q,E23K,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R            1,2,7,8,9,11,12,14,15,16,17,21,22,23,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116        73            
59  PKPEEAMREAYKLIKKYMRKAQKEAQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0059  T1P,R2K,R7M,E8R,R9E,W11Y,R12K,A14I,E15K,I16K,A17Y,G21A,W22Q,E23K,H25A,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R            1,2,7,8,9,11,12,14,15,16,17,21,22,23,25,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116        73            
60  PKPEEAMREAYKLIKKYMRKAQKEAQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0060  T1P,R2K,R7M,E8R,R9E,W11Y,R12K,A14I,E15K,I16K,A17Y,G21A,W22Q,E23K,H25A,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R       1,2,7,8,9,11,12,14,15,16,17,21,22,23,25,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116     74            
61  PKPEEAMREAYKLIKKYMLKAQKEHQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0061  T1P,R2K,R7M,E8R,R9E,W11Y,R12K,A14I,E15K,I16K,A17Y,R19L,G21A,W22Q,E23K,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R            1,2,7,8,9,11,12,14,15,16,17,19,21,22,23,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116        73            
62  PKPEEAMREAYKLIKKYMLKAQKEHQEEWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0062  T1P,R2K,R7M,E8R,R9E,W11Y,R12K,A14I,E15K,I16K,A17Y,R19L,G21A,W22Q,E23K,E26Q,R27E,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R       1,2,7,8,9,11,12,14,15,16,17,19,21,22,23,26,27,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116     74            
63  PKPEEAMREAYKLIKKYMLKAQKEAQREWERMRRTDGTKEEKDMFPEKMIAQALRAIGEIFNAYYWAFLKLQEFKKYPSVRWEEQEEARKRLKIMMKIGAEWAREIAREMKERIKR  test_3lhp_binder_labeled_00002_v0063  T1P,R2K,R7M,E8R,R9E,W11Y,R12K,A14I,E15K,I16K,A17Y,R19L,G21A,W22Q,E23K,H25A,E26Q,W31R,W32M,K33R,A35T,S36D,K37G,G38T,R39K,R42K,L45F,R48K,A52Q,E64Y,W65Y,Q66W,M67A,R68F,E70K,M71L,E72Q,K73E,E74F,R75K,N77Y,N79S,A80V,G81R,E82W,K84E,M85Q,K86E,Q88A,K89R,E91R,A92L,W93K,K94I,I95M,A96M,Y97K,Y98I,W99G,G100A,L101E,M102W,A104R,Y105E,W106I,I107A,K108R,Q109E,H110M,R111K,K113R,E114I,R115K,K116R       1,2,7,8,9,11,12,14,15,16,17,19,21,22,23,25,26,31,32,33,35,36,37,38,39,42,45,48,52,64,65,66,67,68,70,71,72,73,74,75,77,79,80,81,82,84,85,86,88,89,91,92,93,94,95,96,97,98,99,100,101,102,104,105,106,107,108,109,110,111,113,114,115,116     74            

[64 rows x 5 columns]